To increase the levels of pulmonary gene transfer by nonviral vectors, we have adopted electroporation protocols for use in the lung. A volume of 100–200 μl of purified plasmid DNA suspended in saline ...

Cell therapy is an emerging novel treatment option for an array of diseases, ranging from genetic disorders to cancer. As the cell therapy field expands, there is a growing demand for safe and ...

Thermo Fisher Scientific’s new Gibco CTS Xenon Electroporation System aims to provide easier scale up for cell therapies, from clinical development to commercial manufacturing. Thermo Fisher ...

When a cell is able to take up free DNA or RNA, it is termed “competent”. Some cell types or microbial species, such as Bacillus subtilis, 11 are naturally competent and they can be induced to uptake ...

Unleash the power of electroporation with the Invitrogen Neon NxT Electroporation System. Join us for an insightful webinar where we demystify the basics of electroporation and guide you on how to ...

Graduate students Mingde ‘Jack’ Zheng and Joseph Sherba have developed a novel, microfluidic platform for monitoring electroporation and molecular delivery at the single cell-level as part of a NSF ...

Using an electric current to deliver the CRISPR/Cas9 system, in order to engineer genetic changes in laboratory mice, is highly efficient and significantly improves the system's throughput over the ...

CARLSBAD, Calif.--(BUSINESS WIRE)--Thermo Fisher Scientific, the world leader in serving science, has introduced a new large-volume electroporation system that allows cell therapy developers to more ...

In coming up with “A window on new expression,” a couple based in Cleveland, Ohio, provided the answer that solved GEN’s Cryptogram Challenge: Electroporation. Jodi Bubenik, Ph.D., a molecular ...

Knockout mice are animals that have been genetically modified for so that one or more genes are not expressed, or “knocked out.” Studies are then carried out on the mice to determine what the effects ...

3-dimensional rendering of a cell passing through the electroporation zone while a pulse is being applied. The electric field lines which normally do not penetrate an insulating cell are able to ...