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SOLVED: a) In an enzyme catalyzed reaction, when [Et] is 6 nM a Vmax of2 μM*s-1 is obtained. What is kcat? Proper unit must be used.b) In a separate experiment when [Et] is 4 nM and substrateconcentration is 1.5 μM, a V0 of 200 nM*s-1 was obtained. AssumingVmax remains unchanged as in 11) a), calculate Km.
SOLVED: a) In an enzyme catalyzed reaction, when [Et] is 6 nM a Vma…
Feb 10, 2022
numerade.com
SOLVED: Calculate the Km, Vmax, and Kcat for a molecular weight enzyme GOkDa from (final protein concentration in the following "data cuvette IOug/ml) with [S]; mM Vo points: Amol/min10 20 30Calculate Km and Vmax using both Michaelis-Menten data and Lineweaver-Burk equations from the following [S], mM Vo Mmol/min 0.1 0.34 0.2 0.53 0.4 0.74 0.8 0.91 1.6 1.04
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